maruiqi0710 comments

Results 4 comments of


                                            maruiqi0710

Can the database be loaded into memory once to handle multiple paired reads files?

I own this server by myself, how to keep the database from being kicked out of memory. > kraken2=/Software/Bioinfo_Software/kraken2/kraken2 > DBNAME=/home/XXX/Software_Database/kraken2/k2_pluspfp > ######################################################################### > input_dir=/media/clean_data > out_dir=../1_result > file_name=$(find $input_dir...

Can the database be loaded into memory once to handle multiple paired reads files?

I want to confirm the options again. I added the option --preload > $kraken2 --db $DBNAME --threads 60 --output "$out_file" \\ > --report "$report_file" --use-names --preload $input_file , but report...

Do I need to “train” one by one?

> yes you can reuse -- we did this for a pangenome projects - but I found it was helpful to still include the rna alignments for the predict steps...

Which manufacturers' sequencing adapters are included in the reference database of FCS-adaptor?

These sequencing adapters can be found in [(https://en.mgi-tech.com/download/files?q=adapter)](https://en.mgi-tech.com/download/files?q=adapter) . The document titled "**【User Manual】DNBSEQ Dual Barcode Adapter & Barcode Sequences A1**" provides access to these adapters on the aforementioned website....