kiwipeel

TF analysis between conditions scRNA

9

How can I determine if the TF activity between two conditions in my single cell dataset is significant for a gene?

Removing genes from count matrix before doublet detection

3

Hi, I want to remove some rRNA contamination-related genes from my scRNA count matrix. Should I use scdblfinder after or before removing those genes? I know that cell filtering isn't...

I want to analyze only a specific cell type in my data so ı subsetted my data. I did standart seurat workflow and after clusterıng ı realized that some clusters...

How to use our own DEG results ın comparison analysis?

2

Hi, I think tool calculates DEGs between two condition in compatison analysis is at single cell level. I want to import my DEGs results I obtained after doing pseudobulking for...

How to merge biological replicates and do comparison analysis?

1

Hi, I have single cell data with 2 condition and 4 replicates in each condition. In the tutorial why do we use directly cellchat object derived from only conditions? Why...

Hello , Should I use scImpute on different conditions seperately? Or should I use it on merged object directly?