Mian Umair Ahsan
Mian Umair Ahsan
Hi, Currently we have not developed any models for de-novo modification detection, but it is something we plan to do in the near future. We will update the issue once...
Hi, it is reasonable to expect a few reads from FAST5 to not be present in BAM if basecalling fails for those reads. However, this should be tiny fraction of...
Just to add some extra information, yes whole genome bisulfite sequencing and Nanopore sequencing should be sufficient. We have uploaded two CpG models trained from high coverage Guppy basecalled R10.4...
Hi, For CpG, we were able to achieve very high performance (~94% F1) with ~30X NA12878 native ONT dataset using consensus of two WGBS replicates for ground truth, and we...
Hi, It seems like your FAST5 files do not contain move tables. How did you basecall these files?
Hi Jeff, Numba package should have been installed during conda environment creation, but if that didnt happen correctly, you can install it manually by running of these two commands in...
The same commands should also work in LINUX if the numba package is missing.
Hi, yes `-c` is how you specify the model.
Yes, you can use `--device "cuda:0,1"` to specify Guppy to use two GPUs if you have more than one available, similarly use `--device "cuda:0,1,2"` or `--device "cuda:0,1,2,4"` if you want...
Hi, Are you using the latest commit? This bug was fixed in commit 2f7c90daf463a289466c40ff1dac606503cf5f77, so if you use the latest version you should not have this error. Please use `git...