shri1984
shri1984
I did that. I use polca.sh for polshing. I use 1 billion illumina PE reads (150 PE). this is the report Substitution Errors: 991295 Insertion/Deletion Errors: 663392 Assembly Size: 5498989353...
Thanks. I see. I used the options you suggested (-R, aln-dovetail -1 or 1024, -l 500 etc, K 2000) for repetitive genomes (in issue #230). It worked beautifully, but things...
Do you know what is acceptable limit for this reduction? in my case it is 12 %. data is coming from 7 cells of sequel CLR. I am also using...
Hi, I am assembling a large genome. I tried to use -K 2000 after I see high frequency kmer depth set to about 16K. I had 6 cells of sq...
Hi, Thanks for your reply. You are right. It is from an organism with the highly repetitive genome, I tried a few times now. It did not budge. I just...
Thanks again, We are building a transcriptome database covering as many genes as possible. I have a genome with lot of contigs (chromosome level as well as unplaced). Annotation is...
Hi, I have the same issue. md5sum -c checksum.md5 failed (15 cases). I am using a machine with 2 TB RAM (Ubuntu).
Thanks @a-ludi. example data set went fine including the md5sum. The latest version helped. I am trying dentist on my hic scaffolded hifi assembly. I will post the update here.
Thank you very much. I am on it now. Let you know how it goes.
Thank you Shubham.