Joel Hjärne Kokk
Joel Hjärne Kokk
[PySimpleGUI 5 is out, but is now closed-source and subscription-based](https://news.ycombinator.com/item?id=39369353) Although the subscription is free for non-commercial users, which includes most of us, it requires you to acquire a free...
**Is your feature request related to a problem? Please describe.** There are lots of emojis today, most of which I have no clue where they are in the list. An...
### Description of feature The current stop-codon detection in ampliseq could be improved and supplemented with frame-shift detection by implementing [MACSE](https://doi.org/10.1093/molbev/msy159). There are existing Nextflow pipelines [here](https://github.com/ranwez/MACSE_V2_PIPELINES). This is a...
Hi, When working with metabarcoding data where you're not interested in any specific taxonomic group but want to quantify general diversity, you almost always have a mix of different genetic...
Hi Ben, I have a few questions regarding de-novo chimera removal. 1) What is the idea behind pooling samples for chimera removal? As chimeras are formed during PCR, when the...
Hello, I am not an expert, but it seems to me that DADA2's strategy for filtering+trimming+merging doesn't make the best use of the information contained in paired-end data. Perhaps I've...
Hi, It would be great with support for zstd compression and decompression of especially FASTQ files, as they can get very big with modern sequencing technologies, and zstd seems more...
The nbases argument in learnErrors is set to 1e8 by default, i.e. 100 million = 100 000 000 bases to use for error rate learning. Still, I often see messages...
Hi, We routinely take marine bulk samples for metazoan metabarcoding, and when doing so, first we q-tip our equipment and processing station to be able to control for contamination, like...
One feature that sets DADA2 apart from other algorithms is that the recommended workflow is to denoise forward and reverse reads separately, and then merge them. This has always seemed...