Joel Hjärne Kokk

> Having the emoji search in the emoji menue is obvious. Making it only accessible with : will make the function undiscoverable for many people. I could install the keyboard...

Hi @rcedgar! What about `otutab_xtalk`? I have not seen another software with similar functionality of the UNCROSS2 algorithm. Or do you not recommend using it anymore?

> I think cross-talk is too hard to measure accurately, and you cannot filter it without losing too many low-abundance species. I don't use it myself, but it's all a...

> [...] but including control samples seems not to be a widely accepted practice Yeah, unfortunately not. And I don't have any control over what happens in the wet lab...

@d4straub MACSE is an aligner with many applications, one of which is to detect pseudogenes among protein-coding marker genes. Excerpted from the paper: > The _enrichAlignment_ subprogram can be used...

@d4straub - Yes, exactly - Most likely replace - Unfortunately I don't have any experience with Nextflow, and in all honesty not much time either. I use a custom pipeline...

Alright, I see. Let's keep it hanging for now then.

Ok, I see. But wouldn't it be desirable if it did? Break samples up, that is, and actually apply the limit it sets out to apply. What's the recommended way...

Ok. Would be good to have a limit yes :) I'm curious though why it's not desirable when multiple samples are read in? Does the error learning algorithm take which...

Hi Ben, thanks for your response. Does this imply that one should only pool samples for chimera detection if they come from the same environment (e.g. sampling replicates)? And should...