hong.fei

--min-contig-len confused

2

hi sir： i want to get all length contig，with --min-contig-len 0； but the max_len is much small than default parametes. default reuslt as below ``` file format type num_seqxxxs sum_len...

hi sir： i want to get all length contig and reads which donot construct contig， what should I do. thanks

hi sir： i want to use ariba with single-ended reads for same clinic sample， how should I do ? thanks

fabG1 mutation in card web, but not in card.json?

1

hi sir: fabG1 mutation is a very important event in Mycobacterium tuberculosis Resistance，but not in card.json，so RGI will not work on this gene?

hi sir： other resistance tools record Staphylococcus aureus dfrB Mutation resistant to Trimethoprim；but card do not； infomation as below: #Gene_ID Gene_name Codon_pos Ref_nuc Ref_codon Res_codon Resistance PMID Mechanism Notes Required_mut...

there are any variants?variants?

RGI couldn't detect mutation in nocode region

1

hi sir: RGI couldn't detect mutation in nocode region; mutation in promoter regin in Mycobacterium tuberculosis fabG1 confer resistance to isoniazid, RGI disbale do it.

can i use AmpliconArchitect on 30X ~ human bam？

19

hi~ sir： my bams all are more than 30X wiht B37。 Is AmpliconArchitect very slow when run on such large bam？ Waiting for your reply，thanks。

error ！！

5

hi sir： i got a error . can you help me？ #---------------------------- head WGS_A_D_5715_T.somatic.CNV.txt.bed #chr start end cn 12 66449999 66452000 8 18 19847999 20060000 13 18 20059999 20239000 11...

Could ichorCNA be used for low-pass whole genome sequencing of gDNA？

1

In addition to cfDNA, whether the ichorCNA can be used for gDNA？