Results 5 comments of Botond Sipos

@mw55309 My apologies for the delayed response! Unfortunately, sana currently doesn't support FASTQ files with identifiers in the separator lines. I'll update the help message in a PR to clarify...

@mw55309 I started working on parts of the suggested logic. It is a quick hack, but I implemented the lookback to decide the state of the separator lines. You can...

This is likely to be an issue related to the [case (in)sensitivity](https://discussions.apple.com/thread/251191099?sortBy=best) of MacOS file system.

Hi @awesomedeer ! Could you please upload an example input which shows the problem? Best, Botond

Hello! Can you please try using `-N -1` and see what happens?