bam split error

Open jh4yd opened this issue 1 year ago • 1 comments

I had a small pro-virus genome which my fastq files were aligned to. When I tried to split the aligned bam file, I got the error. command line: seqkit bam -N 50000 MBLN01_reads_aln_sorted.bam -o bam_bundles/ output lines: [INFO] Creating BAM bundles from file: MBLN01_reads_aln_sorted.bam [INFO] Minimum reads per bundle: 50000 [INFO] Output directory: bam_bundles/ Bundle Chrom Start End NrRecs NrLoci [ERRO] open bam_bundles/000000000_pHR5604/05:0:9213_bundle.bam: no such file or directory

Do you know the reason? Thank you so much!

Aug 01 '24 17:08 jh4yd

Hello! Can you please try using -N -1 and see what happens?

Aug 02 '24 13:08 botond-sipos