Bin Lu

Thanks. Do you mean that run one round wtpos-cns using long reads, and then one round wtpos-cons using short reads, and finally run other polish tools using short reads? In...

Thanks for quickly reply. Your paper mainly focus on short reads corrections and documented that two rounds is good enough, so I plan to perform one round PacBio long-reads +...

Thanks! I can not find makeidx.pl in spaln installed from conda. Should I re-download from github and compile it? If kindly integrate into spaln should be good for convenience.

I installed spaln from github and worked well. If I want to obtained different format output (like gff, and exon sequences), can I used -O option mutiple times?

Can I set the min length of exon =3 in spaln to avoid this case? You are right, I set the wrong option. Now I can get the right result....

Dear Osamu, I found some sequences header contain "M Deleted 1 chars at 1952597", what that mean? Is it a frameshift? But I can not found stop codon in the...

Thanks. The bed output format like below chr4 10741027 10742890 ARRS_RAT 697 - 10741027 10742890 0,255,255 11 79,138,73,85,136,68,60,194,45,61,60, 0,4776,5105,6812,9839,11412,12819,13008,14229,14398,18573 chr4 10739910 10742890 ARRS_XENLA 988 - 10739910 10742890 0,255,255 15 100,10,56,24,78,138,73,85,136,68,60,194,45,61,60,...

Dear Osamu, When I use the -M option with >1 value, strand info "+" or "-" seems missing in bed file. So I can't determine the strand direction. Please check...