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xsjaxjaxh
Hello! Ragtag is a great software, I am a newbie and I have two small questions. The first one is, "ref1.fasta" and "ref2.fasta" in `ragtag.py scaffold -o out_1 ref1.fasta query.fasta...
Thank you for developing the annotation software BRAKER. I used BRAKER's "https://github.com/Gaius-Augustus/BRAKER/blob/master/docs/long_reads/long_read_protocol.md" to get a pig breed genome annotation file , but the BUSCO evaluation shows that only 65%, this...
Hello! I used the process from "https://github.com/Gaius-Augustus/BRAKER/blob/master/docs/long_reads/long_read_protocol.md". I used ”collapse_isoforms_by_sam.py“ to get the file "merge.combined.gtf", then I ran "stringtie2fa.py" to get the input file "cupcake.fa.mrna", this step works fine. But...
Dear developer Thank you for developing this software，I used the script “get_unmapped_pavs.pl” to extract the sequences on the unaligned reference genome in the BLASTN results and obtained the “genome_unmapped_pavs.txt” file...
Hello! I've successfully gotten the .gamp file of the transcriptome file with `vg mpmap` and he seems to have no problem. But when I run rpvg, he has the following...
Hello! Thank you very much for developing such a good software! I obtained a genome annotation file with reference genome annotation using Liftoff software and then used "gffread" to translate...
Dear Developer Your software is very useful. I have tested it on HPC and it works without any problems. One problem I encountered is that I plan to use assembly...