Matt Cannon
Matt Cannon
Hello! I think this fits here, but if it doesn't let me know and I can create a new issue. I have seen similar issues with timezones when working with...
It's totally possible I'm implementing something incorrectly @ablack3. Databases are outside my normal workflow so I'm not at all an expert. I mostly built my code based on a few...
Hi, can you give me the primers you were searching with so I can see if the issue is reproducible on my end? It's hard to troubleshoot the issue otherwise....
I don't know that it will work that way, but in the past I've used "organism" instead of "ORGANISM" and it works.
Hey, I found a work-around for this: ``` venn_plot
Hey Min Hui, your tree will likely look slightly different as the blast database is frequently updated. So long as your tree looks somewhat similar I think you're good.
I imagine it all depends on what's been added to the database. 2000 is a good starting point, though more is better. At some point you're going to run into...
Yeah, I've seen this before as well. it also can mess up the calc_rank_dist_ave numbers since the genetic distance between sequences within a species/genera can get overestimated. I don't know...
Hey Thiago, that's an interesting idea. The way to do that might be to write a wrapper function to take in primer combinations, run primerTree and then merge the results,...
What primer sequences are you using? I'm guessing something might be off with the primers since it looks to be returning a bunch of 1bp sequences.