Dr. Kendall Lee

How long and how much memory should NextPolish require?

4

**Question or Expected behavior** How long should it take for NextPolish to complete on a ~50Mb long read genome and what memory should I ask for? I submitted it at...

db split1 issue

6

**Describe the bug** Hi I am trying to run NextPolish on Hifi pacbio sequences. I keep running into an unspecified error with db split. My workflow is: ls /scratch/kcl58759/Eco_pacbio_kendall/pb_css_474/cromwell-executions/pb_ccs/c7a3dc30-7f94-40de-ac16-2445f965bfad/call-export_fasta/execution/m64060_210804_174320.hifi_reads.fasta.gz >...

Duplication still >80% after running Hifiasm

6

I ran Hifiasm with default settings. I assumed this would purge the duplications to a max degree. However, after running BUSCO on the primary assembly output, there is a duplication...

ktab.1 is missing when running hetmer

1

Hi, I am trying to follow the template script exactly. FastK seems to have run with no problem but only produces a single ktab file and a single hist file....