王杰

Agree with @johannesgeibel. I have encountered the same questions. My aligning programs by winnowmap always run computing nodes too busy. Is there any better way to change this situation? By...

Hi, @jonassibbesen Sorry for late reply! Yes, file 20_snps_reads.sorted.bam only contains chr1:1-100000 reads because SNPs in these regions are used as test case. So if the problem is that some...

Hi, I'm also using LAST to align my HiFi reads to genomes and encountered the same problem as LiShuhang. My program is running very slowly. When I change the threads...

Thanks for your suggestion. But I don't understand what the -k parameter mean, could you explain it in more detail? Besides, I found that -i parameter can specify the memory...

Thanks a lot! I will read the docs and try the parameters you suggested.

Hi, I have tried the -k and -i parameters using my data. Unfortunately, the improvement of speed was samll. For now, the result maf file can be generated 500M an...

Sorry for not replying to you for a long time! I have tried to split my original file into some small files and one fastq file is 5.2G. Then I...

Yes, I'm using the latest LAST. Here is output of `lastal --version`: ```bash $ lastal --version lastal 1256 ``` You are right. The reason why last run so slow is...

Yes, it's still slower than expected. I tried `-P20 -i100G -k64 -m5 -R00` and the speed is not ideal. It took 54 hours to genearte the 23G file. While `-P20...

Thanks for your considerate replies! Maybe it is the `-i100G` parameter that slows the speed. Since the more sequences it processed, the slower speed the result file is generated. It's...