wingkyBs

Thanks for your reply. I tried popscle dsc-pileup as you said. ___ /path/bin/popscle dsc-pileup --sam ./VF/outs/possorted_genome_bam_hg19.bam --vcf ./HB00007167_VF.vcf --group-list ./barcodes.tsv --out /path/dsc-pileup ___ As a result of this, dsc-pileup.var.gz, plp.gz,...

As you said, I performed popscle dsc-pileup using vcf excluding the X and Y chromosomes and then popscle demuxlet, and no errors occurred! No error occurs when performing popscle demuxlet...

Thank you for your answer. Thank you so much for providing a good program and your quick response. As an additional question, is it okay genotyping using SNG.BEST.GUESS from the...