sunsetyerin

reference files inquiry

1

Hi, can you please guide me on which reference file to use for direct human RNA? For nanopolish aligning, should the reference file be genome or transcriptome? Also, for xpore...

eventalign.txt file size

3

Hi, for the data preparation, I followed xpore docs. My data is direct RNA and used gencode transcriptome fasta file for the reference. And received 2T eventalign.txt files for each...

eventalign.txt.gz file as an input

1

hi, I generated 2TB eventalign.txt using nanopolish as a result. Since it's nonsense storing it, I gzipped it and when I tried to apply xpore dataprep for the compressed eventalign.txt.gz,...

nanopolish polya with guppy 6.0.6

1

Hi @jts Is it possible to "nanopolish polya" with guppy 6.0.6 called fastq files? Or do only albacore basecalled fastq files work with the command? Seen from the documentation, it...