Sam Kovaka
Sam Kovaka
I may have figured out a solution to this! It looks like the array pointer remains valid as long as the `py::buffer_info` object returned by `buffer.request()` exists. I've written a...
Try running the command `~/.local/bin/uncalled`. If that works, then you could add the command `export PATH=~/.local/bin:$PATH` to your "~/.bashrc" file to add that directory to your system path. If that...
This should be possible via the `uncalled convert` command. Currently the only way to compare with the projected guppy alignments is to additionally run `uncalled dtw` and write to the...
Ah yes, sorry I forgot resquiggle refered to aligning to the basecalled reads. That will take some reworking to handle reads not aligning to a common reference, but it does...
I do support BAM move tables now. You just need to run Guppy with the "--moves_out" and "--align_ref" options, merge the resulting BAM files with samtools, then input the merged...
UNCALLED does not require a GPU, and it's compatible with MinION and GridION. However, unfortunately we currently can't handle the full human genome, as we are limited to sequences less...
When you say a few thousand reads, out of how many? If it's 1,000 out of 1 million, for example, that's a 0.1% error rate, which is about as good...
Sorry for the issue! I'm not sure what's causing it. I don't think it's the same error as #22, since that segfaulted immediately after loading reads, while yours looks like...
Hi, Are you using the Zymo High Molecular Weight pre-extracted DNA, or a different Zymo product? Either way, I'd suggest measuring the read lengths of Saccharomyces and bacterial reads separately,...
What type of flowcell are you using? UNCALLED only supports r9.4.1, which is now a "legacy" kit. That was sort of buried in the README, so I just added a...