Santosh

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Hi Vadim, Thank you for the quick response. I was wondering to generate the refined library using FASTA predicated (.predicted.speclib) when the real samples dia-PASEF files are loaded. Does this...

But if you see the above screenshot, the option of generate spectral library is not enabled.

So should I restart the search again

Okay. Thank you. So, do you recommend to search the .d files again with the refined spectral library (builded out of dia files)? Thanks, Santosh

Thank you Vadim :)