ljy-sys

> TRUST4 only assembles the first portion of C genes, maybe around 200bp. To get those sequences, you also need the AIRR format, and utilize the "sequence" column and the...

> I forgot to mention that the header i the _annot.fa file in the smartseq wrapper also contains the coordinate for the C gene, which probably is more accurate than...

> Do you mean you also need the amino acid sequence including V and J genes? In this case, you shall use the AIRR output format, and translate the amino...

> Do you mean you also need the amino acid sequence including V and J genes? In this case, you shall use the AIRR output format, and translate the amino...

> The smartseq.pl wrapper shall also output the _airr.tsv file. The sequence_alignment column includes how the underlying full sequence aligned against the IMGT database (germline_alignment column). But you may need...

> "sequence" column is the underlying assembled contigs, which includes regions outside of the V genes. The "sequence_alignement" is the portion that can be aligned to the IMGT database, so...

One more question, does RunHarmony involve random algorithms? Why are the results different between the two runs?

> Thanks for this tools to help us analysis the smartseq3 data，but when i using the parameter “additional_files” to add a exogenous sequence，the result without this sequence is not a...

> Hi, > > Sorry I do not understand the issue, what do you mean with "the result without this sequence is not a umicount of 0"? En, this means...

> Hi Sqjin, > > I am doing comparison of multiple cellchat dataset. For each, i finished running to cellchat > Do you know how to solve this? > >...