multiPrime
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joybio
multiPrime is a mismatch-tolerant minimal primer set design tool for large and diverse sequences (e.g. Virus). Here is a web-based version (test: http://multiPrime.cn))
Here is my output:  Here is my input: 
Hi, I have tried the https://multiprime.cn/#Home page, and my fasta cannot be uploaded, I checked there are no : , _, |, ", or space in my fasta.  Encloesd...
 I attempted to upload several sequences, but encountered no response. What could be the issue?
my input:  my cluster: 
Your paper and code are quite interesting and inspirational. This is not a question about the code, but I request it here to get a faster response. The S2 figure...
Hello！I have some errors when running the program, I would be grateful if you could take time out of your busy schedule to answer my doubts.Pic 1:Is it because of...
Hello, I ran the following code, and there were no errors during the execution. However, the resulting file is empty. Why could this be? code: `python scripts/multiPrime-core.py -i workspace/inputs/MICrhoDE_dna_aligned.msa -o...
MissingInputException in line 43 of /home/data/t220349/yuzh/multiPrime_test1/multiPrime/multiPrime.py: Missing input files for rule seq_format: output: yuzh/multiPrime_test1/multiPrime/results/Total_fa/1000.format.fa wildcards: virus=1000 affected files: yuzh/multiPrime_test1/multiPrime/test_data/1000.fa When I run to this step, an error is reported, I...
 Hi, I download the multiPrime2-GUI，but can't install successful. Failed to execute script 'multiPrime-core_GUI' due to unhandled exception: cannot unpack non-iterable NoneType object.
