Duroc the pig

Does a low `unspliced` proportion have an impact on velocity calculation ?

1

Hi, I have many germ cell scRNA datasets, and all of them have a very low `unspliced` proportion ( about 5% to 10% ). The `velocyto` pipeline gave me a...

velocyte `run10x` error: Genome annotation gtf file is not sorted correctly!

2

I use my customized `.gtf` file to run 10x scRNA pipeline and everything is OK. However, when it came to velocyte `run10x`, here is an error: ``` OSError: Genome annotation...

I have a large dgCmatrix object made by `Read10X()` function. ``` mtx

Error in `Convert()` an `.h5Seurat` object into `.h5ad`.

1

I am trying to convert a seurat object into `.h5ad`, and here is my `data` and code: ``` data An object of class Seurat 35282 features across 5366 samples within...

How could I know the Process State of Hic-pro?

2

Here are some problems in one of my sample fastq data , so the mergeBAM.py stopped. And the /hicproout/logs/ directory seemed to have no change any more. I am not...

Hi everyone, it's not an issue but a discussion. I have a pfmlist object `head()` like this: ``` $M00373 [,1] [,2] [,3] [,4] [,5] [,6] [,7] A 0.4068930 0.57303098 0.976367797...

Hi, I download the vignette data `muo_data.rds` load the `muo_data.rds` as the object `seurat_obj` and running the vignette here is my code ``` seurat_obj