Litt

issue with split_on_adapter output

17

I successfully ran split_on_adapter with the --allow-multiple-splits option and it split a bunch of reads for me. When converting the new mapped SAM file to BAM format, it wrote about...

More RNA mod models?

5

The DRACH motif 6mA model is amazing!!! Are there going to be models coming out for pseudo-U and inosine?

Foldback chimeras

5

Is there a way to get rid of duplex foldback chimeras? It doesn't seem like Dorado splits the + and - strand of duplex reads into two reads. I mean...

pod5 support?

15

will pod5 format be supported?

Smart-seq2?

3

I'm a little confused on how this pipeline works: 1. Why does the preprocessing separate each input BAM into chromosomes? I'm trying to call variants genome-wide. 2. Why does it...