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Same problem. I installed primer3 through brew, ran vcfkit as the following: vk primer sanger --ref=genome.fa test.vcf.gz I get no error and no output too.

I never could get nr to build with the provided command. I did follow the process outlined here (https://blobtoolkit.genomehubs.org/install/ ) to setup uniprot which worked fine though. I'm using nt...

What is the issue with the HIFI data? I noticed the kmer plot doesn't look like the bimodal distribution. Is it because I'm providing samples from both male and female...

I was hoping to get the male/female specific chromosomes, but I could just do two assemblies and add the specific chr to the other assembly. Either way, I just tried...