Clint
Clint
Scan from 5' end to find a given sequence, then trim that sequence AND everything to the left. This is useful for times when you are sequencing amplicons and you...
@sfchen - Did my clarification make sense? (I know it's been a while, but I wanted to see what you think about this feature.)
You bet. This is a build using 811 sequences. The results/aligned.fasta are identical (diff) ,but all the files in results/global differ. That includes the subsampled_alignment ans sample-global fasta files--although the...
Good suggestions. I reran the tree and refine steps on the alignment several times, and what I got was that they come out differently every time--about half create that outgroup....
I just discovered this issue after a certain dataset was really giving surprising results. Off target amplification products give short (20-25 base) alignments to a given reference sequence really screwing...
I actually think it would be a better default to have the report generation off, rather than default to a single filename which conflicts with parallel fastp processes. The user...
Hmm. I don't think it is actually doing anything recursively... ``` $ poretools fastq --group 001 workspace/pass/*/ > reads_001.fastq WARNING:poretools:Cannot open file: workspace/pass/0/. Perhaps it is corrupt? Moving on. WARNING:poretools:Cannot...
Yes, that works much better :+1: Thanks!!
I also have wondered what the y-axis means with these plots, and I thought I was missing something. It is wacky, and always has high numbers. I just replicated this...