Connor T. Skennerton
Connor T. Skennerton
You can. There is a small risk that some CRISPR will not be detected in the split files but they would be rare in the data. > On Apr 20,...
You are correct that Spacers_6 should be the spacers from group 6 and the sequence should be the DR sequence. It's been a long time since I've developed this code...
I think you've actually run crass multiple times, once for each of the input files in $READDIR. If you are intending to run crass once on all of the files...
I think if the files are all from one sample ⏤ like R1 and R2 files from Illumina generated data ⏤ then they should be run together. If they are...
Which SRA run are you using? When I search for SRS140663 I get multiple results
I think it's doing the same thing on my machine. Interestingly it's in the final xml file writing step, which has never been a problem before. I'll see what I...
I'm not quite sure what the problem is but the number of identified reads in the sample you sent is huge (~7,000,000 of ~10,000,000). I've never seen so many reads...
It does appear to be eukaryotic contamination. I don't think there is a good way to detect this systematically but I'll put in a test that if too many reads...
When I looked at the file I saw many reads that appeared to have a poly-A tail - they also appeared to be very similar to each other (i.e. many...
What version of crass are you using? > On Jul 15, 2016, at 11:05 AM, Shaman [email protected] wrote: > > Hi, > > I attempted running crass on my data....