Chris Fields
Chris Fields
We're just getting in our first standard 16S data from the PacBio Revio which is using the [Kinnex kit](https://www.pacb.com/technology/kinnex/), and will soon be getting 16S-ITS-23S data. I know the FASTQ...
Just a general question. We're testing using DADA2 for PacBio amplicon data to compare it with other methods for allele and copy number analysis. During troubleshooting I did find there...
The RStudio example seems to crash when I try testing it. When downloading and trying this locally I ran into several issues with dependencies such as `rlang`, which required manual...
When running extract_discordants.py on our cluster, I noticed two issues, the first pretty severe. Any ideas? - When extracting the original (query-ordered) BAM file, a *.bedpe file is generated. However,...
We can start pushing releases to CPAN, completely missed this: https://docs.perl6.org/language/modules.html#Upload_your_Module_to_CPAN
Stacking roles (using roles within roles) doesn't seem to work when attributes are required, even when the 'with' keyword is used after attributes are declared. See the Biome::Role::Location::Does_\* classes (interfaces),...
Hi @benjjneb this isn't an issue with dada2 but more a general question. We're using a slightly modified version of the big data workflow implemented in Nextflow for processing PacBio...
Would it be possible to tag a new release for the recent updates for the binning publication? https://genomebiology.biomedcentral.com/articles/10.1186/s13059-024-03234-6 The paper mentions r73, but the latest tagged release is r63.2. We're...
*Describe the bug* FCS description for contaminant is not present in UniVec *To Reproduce* Not applicable, FCS run completed. Question is with output and missing data from UniVec database *Software...
I'm using an approach like that described in the 'Big Data' workflow, but with the `dada` step in the loop farmed out to independent worker jobs on a cluster so...