Ali May

HINGE process getting killed with large datasets due to single large las file (?)

2

Hi guys, Recently we've realised that the way we run the HINGE pipeline causes a process in the chain getting killed for large PacBio and ONT datasets. I think this...

HINGE graph output

3

Hi guys, Is there way to convert the HINGE output to a format that can be used for [Bandage](https://github.com/rrwick/Bandage)? A format like spade's fastg? Thanks in advance, Ali

Hi guys, Just wanted to let you know that last week I used HINGE with low-coverage parameters suggested by Ilan [here ](https://github.com/HingeAssembler/HINGE/issues/104#issuecomment-288573948) on 9,567 MinIon reads (min. length 8 Kb),...

HINGE with PacBio CCS reads

5

Hi all, I've been trying to run HINGE using Circular Consensus Sequencing reads that are obtained after a Sequel run. As expected the number of reads decreases drastically due to...

Congratulations on the paper!

2

You made it! Very nice!

microbiome::transform confusing transformation

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Hi, thanks for developing the microbiome package. Question about microbiome::transform(): When I provide the function with a phyloseq object where rows are OTUs and columns are samples, and run it...

0 Bracken added reads with GTDB database for all taxonomic units

4

Hi there, Thanks for developing Struo2 and for releasing the GTDB kraken2 dbs prepared with struo2. I'm using Kraken v2.1.1 and Bracken v2.8 on paired-end animal microbiome data and the...