Agustin Baricalla

Did you try ``` singularity build mitohifi.sif docker://ghcr.io/marcelauliano/mitohifi:master ``` ?? Then use ``` singularity shell -e mitohifi.sif mitohifi [opts] [files] ``` Or ``` singularity exec mitohifi.sif mitohifi [opts] [files] ```

same problem here, not python2.7 nor python3.11 work with this. Hope there is a quick solution

Hi there! I don't know all the answers to your questions, but for the question: 'Would a higher or lower coverage target for down-sampling be more recommended than 100X?' There...

Hi @JohnUrban . A few suggestions would be: 1- usegalaxy.org or **usegalaxy.eu** = a public platform with Verkko, Hifiasm, and Flye. There are no RAM problems, as you are having...

Hi @Isoris Certainly you can use [flye as a polisher](https://github.com/mikolmogorov/Flye/blob/flye/docs/USAGE.md#running-only-flye-polisher) but usually this is reserved for HiFi reads. It would be best if you tried with the ONT reads or...

Hi @ap1438 ! You should try the singularity version, no root privileges are needed. e.g.: quay.io/biocontainers/hifiasm Hope this can help you.

This question was replied to in a different context, but certainly this answer should help you: #https://github.com/chhylp123/hifiasm/issues/849#issuecomment-3019737180 "We’re planning to release a new version soon that can directly assemble both...

Hi @huangqikai If you have hic data you should try to add this data to the parameters and use the '--n-hap 4' option. Best.

Hi @Jokendo-collab. With such a huge amount of coverage that you have, could you better use the longer reads and reduce the coverage to 50-100X? Did you try to `--rl-cut...

Hi @nickgladman I'm a user of hifiasm for a while now and I was watching your log file and usually the homozygous peak is approximately the half of the heterozygous...