zwei.liu

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Thank you @tomasbruna , it can go through this step after I updated braker2 to 2.1.6 and prothint to 2.6.0. But then a new error happened, which looks as follows...

> It will be easier to debug this on [test3.sh](https://github.com/Gaius-Augustus/BRAKER/blob/master/example/tests/test3.sh) to figure out whether the issue is with your installation or your input data. > > You can find the...

> The low number of DIAMOND is almost certainly causing the error. > > OrthoDB seems to be very sparse around _Penaeus Vannamei_ (no proteins in the same taxonomic order,...

> You can also try looking for more closely related protein sets (outside of OrthoDB). We are recommending OrthoDB because it covers most species pretty well, but BRAKER will work...

I met a same problem

> Try this one: > > ```r > x pAdjustMethod = "BH",qvalueCutoff = 0.05) > ``` It works, thanks!

Yes. Here is my command lines: ``` for i in *.chr.bam; \ do \ i=${i%.chr.bam*}; \ nohup ROSE_main.py --custom *_refseq.ucsc -i ${i}.cut.bed -r ${i}.chr.bam -o ./${i}/ 2>${i}.log & \ done...

> Dear Liu, > > Thank you so much for your detailed reply. > > However, I encountered an error with gff3ToGenePred. The error message is: 'CDS feature must have...

I found that if i run my command without `-i` option then it works properlly: `gff3_QC -g Lvan.LG.deduplication.gff3 -f Lvan.genome.fasta -o report.txt -s statistic.txt` But it is neccesary for me...

@mpoelchau OK, I can send you my fasta and gff3 file. Please tell me your email address.