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RobertsLab
In between your lab tasks or on days when you work remotely, please spend some time reading papers and performing literature searches for oyster research in the following areas: -...
Is there a way to develop an assay such that one could compare different oyster cohorts on a farm that would provide and stress index/approximation. In other words these are...
Assess Resazurin exposure and signal in open and closed oysters
RNA extractions and qPCR for Lifestage Carryover project for 60 snap frozen samples. These can be done in the same way as the previous round for the same targets (ATP...
Conduct experiment to analyze effect of pre-stress on resazurin response. - Expose oysters to varying levels of temperature stress prior to resazurin measurement - Measure metabolic response for each individual...
Many of the timeseries P.evermanni samples have extremely low read counts (which is expected, based on Azenta sequencing report). Several also have very *high* counts, much higher than any of...
Roberts SB, Goetz G, White S, and Goetz F (2009) Analysis of genes isolated from plated hemocytes of the Pacific Oyster, Crassostrea gigas Marine Biotechnology 11:24-44. doi: 10.1007/s10126-008-9117-6 We should...
Replicate mechanical stress experiment increasing sample size to 8 mechanically stressed oysters and 8 control oysters. Be sure to record observed condition of oysters and size.
Run resazurin trials on USDA seed (5 families) to test for genetic variation in responses. Temperatures: - Control (20°C) - 36°C (metabolic stimulation observed) - 42°C (metabolic depression observed) Sample...
PO submitted to Azenta - I'll now start working on extractions using Zymo Quick DNA/RNA miniprep kits.