primerTree

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Hello, Setting the `num_aligns` option of a search_primer_pair query above 1000 throws up the error > Error in curl::curl_fetch_memory(url, handle = handle) : >> Error in the HTTP2 framing layer...

bheywar

when using the function `primer_search` providing a database of sequences (ncbi query) and a pair of primers (forward and reverse) the results provided by primer tree can include For-For and...

Sigma88

On the construction of Mammmal 16S primer tree

4

Hi, I am currently learning to use the PrimerTree software package developed by your team. When I constructed a phylogenetic tree of 16S mammal primes with the following script, the...

Minhuidong

howdy! I talk with u because i wanna know what are the extra functionalities that are in the ncbi primer-blast webpage and how apply it to the code. Thank u

perrochico-madefoker

Degenerate primers | undefined columns selected

6

Hi, I am trying to check the specificity of the following degenerate primer pair from the literature. Unfortunately, it is not producing any results. BLAST alignment processing, refreshing in 20...

thierryjanssens

Error in curl

9

Hi, I'm trying to use the function ``` search_primer_pair ``` and keeps returning the error: ``` ... Error in curl::handle_setform(handle, .list = req$fields) : Unsupported value type for form field...

saulobritto

error in calc_rank_dist_ave

2

Hello again! I'm trying to use calc_rank_dist_ave command, but it returns the following error: Error in names(x)

babinecha

Use more than one forward and/or reverse as input for search_primer_pairs()

2

Hello, everyone! This is an incredibly useful package and I've been using it a lot since last year. Recently, I started designing my primers using the DECIPHER package. It returns...

tjacomasso

Primer-Blast parameter ORGANISM is not recognised

4

Hi again, kkkkkk I would like to restrict the search blast to a specific organism, it is possible in primer-blast ncbi interface. However, in primertree, an error is reported when...

rogeriobioinfo

clustal_options variable for filter_seqs

2

Hi PrimerTree developers, I've come across a slight annoyance with the filter_seqs function, where if you have a custom clustal omega installation you will get an error: `Error in system(paste(exec,...

alexpiper