JamalEH
JamalEH
Hi Alex, I experienced exactly the same thing in my datasets (polyA+ unstranded RNA-seq datasets), but I never get feedbacks on these subset of abundant and highly significant events. I...
@timbitz Thank you so much for your feedback! Actually, I observed a similar trend of TS/TE events frequency in a dataset of 150M reads per library, on average. The dataset...
Hi Zheng, I did not solve the issue, and unfortunately I got no feedback from the authors so I just stopped using whippet due to this lack of documentation and...
Dear Zheng, Sorry for the late reply! I think if I understood the concept behind splicing analysis of Whippet program is that the tool reports an exon cassette event as...
Dear Zheng, Thak you for your reply! So basically you would consider the TE events, the predominant events in your dataset, as true events? Kind regards, Jamal.
Dear Zheng, I have observed in the dPSI output file that some significant events have length of 1 nucleotide (end_coordinates - start coordinates of the significant node). Did you observe...
Hi Zheng, Thank you for your reply! Those events are still the most abundant and significant in my dataset even if increasing the filtering creteria (Posterior P > 0.95 and...
Hi Zheng, Thank you so much for you reply! Ideed, you are absolutely right, those very short events are maybe cryptic or microexons, but I'm not sure they could also...
Dear Eduardo, Thank you so much for your reply! So even if the single genes are present within the GTF file there is no AS event will be assigned to...
Dear Eduardo, Thank you so much for your prompt reply! When including the strand information like this: chr18 63127035 63128759 - BCL2_E1 chr18 63123346 63127034 - BCL2_E2 chr18 63126835 63127035...