ETaSky
ETaSky
Hello, I was wondering if the behaviour of -p has been adjusted according to these comments in QIIME-1.9.1? I am having some weird results (filtering out a lot of sequences...
Thanks, @alk224 and @jairideout! Regarding the read quality, I actually have checked a test sample, after the merging, 99% of the reads have Q20 or higher. So it is not...
@alk224 Thanks! Yes, the first few sequences in the file seem to have a length of 253 bp. I suspect I had small PCR artefacts. I was not the one...
@alk224 Thanks! I checked by using just Read 1. But the results are really hard to tell. This dataset has around 4 million reads, and about 1 million were filtered...
@alk224 Thanks! And sorry for off the topic unintentionally. Please delete my irrelevant post, if necessary.
exact same issue here in QIIME 1.9.1. Have you figured it out yet? I guess it is related to the category of mapping file.
OK, just found a solution mentioned in an google group post. Fill all empty space in the mapping file with NA (or something). Mine used to have those Description, Barcode...
> @carandraug and @cjfields, thanks for the replies. I am well aware of the intention to break up the monolithic package into smaller, more manageable packages, which I applaud. I'm...
@msevi @jackchen129 My understanding is it is the 85_otus.fasta file, aka, the rep_set that has been clustered with 85% identity.
I have the same error and after doing some research, I think the issue might be multifaceted. Regardless, the core issue is that the `request.get()` command in `retrieve()` function of...