Dario-Rocha

well, I need to move forward with my project so I need downgrading for the moment, Could you be so kind, @dango147, to share the older Seurat and SeuratObject libraries...

Somehow can't downgrade the Matrix package, also I am on a mac and the binaries you kindly provided are for windows, thank you a lot for the effort anyway! hopefully...

I've just tried after updating to seurat 5.1.0 and the issue is still the same on a 1.3 million cells and 92 samples dataset.

Thank you for your reply, however I can't manage to do this because > Error: Error opening file: matrix_bp_soup/index_starts Maybe this is a consequence of the expression matrix in question...

After updating BPcells, the error is now indicating that too many matrices are opened. Indeed, this seurat object was created from a list of 92 BPCells matrices. I have read...

Hello there, although this is not the exact same issue I've decided to reply here because it's basically the same thing, just with a different error I have a seuratv5...

Hello again, You've always been so attentive and I come again to you asking for help. I am commenting on this closed issue because I am encountering the same problem,...