Cole Lyman

Thanks for using CRISPResso2 @Rajeefa! Would you mind posting the version of CRISPResso2 that you are using? Also, it looks like there is an error reading the gene annotation file....

Thanks for the information. Do you happen to know if the file `gencodev41.gz` is in the current directory? If not, try specifying the path to the file. Also, would you...

Thanks for the update, I think there are a few ways that you can proceed: - You can add the `--no_rerun` option to not recompute any steps that have already...

@kclem I have fixed the bugs in the `get_consensus_alignment_from_pairs` function (according to the test cases and running the `nhej` example), but now I get an error here https://github.com/edilytics/CRISPResso2/blob/upstream-paired-processing/CRISPResso2/CRISPRessoCORE.py#L2619 It is...

Also, if the error persists could you provide the output with the `--debug` parameter added?

I suspect that this is happening because the alignment is requiring too much memory. Do you happen to know how much RAM your computer has?

Hi @JH1606-code, Thanks for using CRISPResso2. As you have discovered we currently don't support multiple pegRNA, pegRNA extension sequences or ngRNAs. However, this is on our roadmap to implement. You...

Thanks for using CRISPResso2! I don't think this is currently implemented, but seems like it would be a neat feature. @kclem may have more ideas on how to currently do...

Thanks for the helpful comment @mikeckennedy! @Snicker7 and I are working on this together. Here is what we came up with and seems to work. ``` def render_template(template_name, jinja2_env, **data):...

Hi @clarazourray, Thanks for using CRISPResso2! This seems like a strange error. It doesn't look like there is an error with your reads, but I suspect there is an issue...