BenNiLu

Problem with pysam: ValueError: fetch called on bamfile without index

1

So I'm trying to run clear_quant on a RNA-seq file I obtained online (Ribo-), but I cannot seem to move past the following error: > ###Parameters: > Namespace(bowtie1='/home/nilu/Alignment_db/base_files/ensembl/bowtie/bowtie_index', genome='/home/nilu/Alignment_db/base_files/ensembl/base_files/Homo_sapiens.GRCh38.dna.primary_assembly.fa', gtf='/home/nilu/Alignment_db/base_files/ensembl/base_files/Homo_sapiens.GRCh38.109.gtf',...