Albertperlas

hi, @chrishendra93 don't worry at all about your late reply. The size of the eventalign file is 1.2GB. The command used was this one: `nanopolish eventalign --reads ./concatenatedHAC.fastq --bam ./output.sorted.bam...

Hi, @chrishendra93 , sorry for my late reply. I have tried to use dataprep with --min_segment_count argument to 1, however, I found the same: data.info and data.json empty. There is...

Hi, Thanks for the suggestion. I have run nanopolish with the additional parameter: --samples, and used the output .txt for m6anet dataprep but now this error appears and the job...

> Hi, @chrishendra93 , sorry for my late reply. I have tried to use dataprep with --min_segment_count argument to 1, however, I found the same: data.info and data.json empty. There...

hi @chrishendra93, I will describe all my steps, let's see if you find any errors: I have sequenced a sample with a high concentration of avian influenza virus, a segmented...

Hi @chrishendra93, I also share with you one of my fast5 files in case you want to test alternative options: https://drive.google.com/file/d/1RrYReeM6AWNmPJLBx6LfntSkYNLhbm10/view?usp=sharing Thanks again, Albert

Hi @chrishendra93 , here you can find all fast5 files, the concatenated fastq file after base calling (**max_vRNA.fastq**), the filtered fastq file after removing short reads

Hi @chrishendra93, sorry to bother you again with this, but do you have any news on this issue? Thanks!