Herry

bwa index problem when indexing files with large size

8

I have downloaded the nt data base from NCBI , for which the size is about 90G, I want to create a index file with commands as follows: bwa index...

Dear all, I want to try if RoseTTAFold can well predict the protein complex such as virus-host interactions. So I used the protein sequences of SARS-CoV-2 RBD and human ACE2...

Issues on running independently

2

If I have already clustered the unaligned nucleotide sequences manually and only want to design primers for these clustered fasta files seperately, which script should i use? And if i...

mfeprimer-3.3.1 takes too much memory space!!

2

How to release the memory space taken by mfeprimer-3.3.1? I want to analysis the specificity of my primers which contain almost 50000 18~24bp sequences while the task always broke down...

Why mmseq2 is much slower than blastn?

10

Hi, friends, I want to align some nucleotide sequences to my references. So i tested the mmseqs easy-search and traditional blastn using shell command 'time' while i found the running...

Hi, wubin. I didn't find the "Product_Tm_°C" and "Annealing_Temp_°C" information in the *.spec.tsv outcome file (mfeprimer spec result) which are pretty meaningful for some analysis while i do find this...