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remove PCR duplicate

Open yx-xu opened this issue 3 years ago • 2 comments

Hi! I'm sorry to bother you. I followed Ribocoed workflow, but I don't know whether need to remove PCR duplicate after STAR alignment. Could you give me some suggestions? Thank you very much!

yx-xu avatar Jul 21 '22 07:07 yx-xu

If you only want to identify the novel translating ORFs, PCR duplication removal is not necessary.

PCR duplications might influence the quantification of the translational level of ORFs。

zhengtaoxiao avatar Jul 29 '22 01:07 zhengtaoxiao

Get it! Thanks for your reply!

yx-xu avatar Oct 06 '22 03:10 yx-xu