vg
vg copied to clipboard
vg rna for gene seqwish file?
Hello,
I created a graph genome for individual gene of interest. Now I want to create a spliced graph to create a pan transcriptome to ultimately use it for vg call. For now, I have created a merged GTF file (for entire genome) from all the RNA seq samples by alignment and making individual GTF files.
To run vg rna, do I need to use chromosome-wise seqwish graph or can I use seqwish graph made for individual gene? Thank you!