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vg rna for gene seqwish file?

Open GSgen opened this issue 3 years ago • 0 comments

Hello,

I created a graph genome for individual gene of interest. Now I want to create a spliced graph to create a pan transcriptome to ultimately use it for vg call. For now, I have created a merged GTF file (for entire genome) from all the RNA seq samples by alignment and making individual GTF files.

To run vg rna, do I need to use chromosome-wise seqwish graph or can I use seqwish graph made for individual gene? Thank you!

GSgen avatar Feb 16 '22 15:02 GSgen