plass

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Paired read prediction - mergereads failed

3

## Expected Behavior Hello, I am trying to run PLASS on a curated set of marine viral metagenomic reads. I have two read files, and I am trying to run...

dnolin13

Install issues

6

## Expected Behavior I'm having issues installing plass using all options. I work in CentOS 8 x86_64 ## Current Behavior 1. Install from bioconda appears to succeed but then the...

slschnorr

Use PLASS in metatranscriptomic data

5

Hi I have 2x150 bp metatranscriptomic reads (prokaryotic) and I'd like to use PLASS to assemble proteins. Should it be used the same way as for a metagenome? In this...

jjsanchezgil

Empty (len:0) sequences in plass output

4

## Expected Behavior Do not write sequences in the output shorter than `--min-length`, which is 45 aa by default. ## Current Behavior Sequences shorter than `--min-length` are being written in...

aleixop

Using more stringent parameters to avoid spurious sequences

9

Hey there, I've been testing some PLASS assemblies with my datasets and I noticed that it retrieves ~50x times more proteins than my MEGAHIT+Prodigal predictions, which is way more than...

apcamargo

Some Issues about the length of protein sequences

2

Hi there, Here I'd like to thanks for this excellent tool for assemble short read sequencing data on a protein level, it improved the utilization of reads to a large...

susutBu

MPI local-tmp

1

Hi does PLASS have the option for `--local-tmp` like in MMseqs2 for MPI jobs? I checked the Thanks Antonio

genomewalker

The output file is empty

1

I have one nucleotide fasta file (nuc1.fasta) and I run the following command plass assemble nuc1.fasta result.fasta tmp from my understanding the final output should be result.fasta but the result.fasta...

Sami-Altayyar

Use Plass for euk metagenomics data

2

I want to extract euk genes/proteins from metagenomics data. I want to build a gene/protein catalog for euk genes. Seems that metaeuk is a reference guided approach ( based on...

liuxianghui

Error running mpi job with class

1

Dear sir: I have no problem running a single plass job on my Linux cluster. However, I want to try for mpi plass job. Here are the details for the...

liuxianghui