no outputs for speciation

[qinghong2019]

Hi, I run "run_midas.py species" with the default database with gut metagenomics and fastq from SRR13836152 (human gut microbiome). no results at all. everytime it has the message: MIDAS: Metagenomic Intra-species Diversity Analysis System version 1.3.0; github.com/snayfach/MIDAS Copyright (C) 2015-2016 Stephen Nayfach Freely distributed under the GNU General Public License (GPLv3)

===========Parameters=========== Command: /homes/qinghong/miniconda3/envs/midas/bin/run_midas.py species -d ../MIDAS_ref/midas_db_v1.2 ./test -1 SRR13836152.fastq Script: run_midas.py species Database: ../MIDAS_ref/midas_db_v1.2 Output directory: ./test Input reads (unpaired): SRR13836152.fastq Remove temporary files: False Word size for database search: 28 Minimum mapping alignment coverage: 0.75 Number of reads to use from input: use all Number of threads for database search: 1

Aligning reads to marker-genes database 0.18 minutes 0.74 Gb maximum memory

Classifying reads total alignments: 0 uniquely mapped reads: 0 ambiguously mapped reads: 0 0.0 minutes 0.74 Gb maximum memory

Estimating species abundance total marker-gene coverage: 0.0 0.0 minutes 0.74 Gb maximum memory.

I know the default database is human gut microbiome, it should return some results. any help? thank you

[Arch0nnn]

/ @qinghong2019 Hello, I encountered the same problem as you. I used intestinal microbiome data and the output on run_midas.py species also had no results. How did you solve it? Thank you very much for your reply.