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pasa-assembly problem

Open DiegoSafian opened this issue 2 years ago • 2 comments

Are you using the latest release? yes

Describe the bug I have been running several funannotate update jobs in parallel and I get an error in earlier steps. It is strange because sometimes the sudden error happens and sometimes does not happens and the jobs are finished without error. I wonder if this is related to the jobs running in parallel.

Error: [May 19 04:03 PM]: CMD ERROR: /camp/home/safiand/home/users/safiand/.conda/envs/funannotate/opt/pasa-2.5.2/Launch_PASA_pipeline.pl -c /nemo/lab/cardoso-moreiam/home/users/safiand/genome_annotation/prolifica/funannotate/update/update_misc/pasa/alignAssembly.txt -r -C -R -g /nemo/lab/cardoso-moreiam/home/users/safiand/genome_annotation/prolifica/funannotate/update/update_misc/genome.fa --IMPORT_CUSTOM_ALIGNMENTS /nemo/lab/cardoso-moreiam/home/users/safiand/genome_annotation/prolifica/funannotate/update/update_misc/trinity.alignments.gff3 -T -t /nemo/lab/cardoso-moreiam/home/users/safiand/genome_annotation/prolifica/funannotate/update/update_misc/trinity.fasta.clean -u /nemo/lab/cardoso-moreiam/home/users/safiand/genome_annotation/prolifica/funannotate/update/update_misc/trinity.fasta --stringent_alignment_overlap 30.0 --TRANSDECODER --MAX_INTRON_LENGTH 3000 --CPU 20 --ALIGNERS blat

What command did you issue? funannotate update

Logfiles Please provide relavent log files of the error.

pasa-assembly.log

DiegoSafian avatar May 19 '23 18:05 DiegoSafian

SQLite is single threaded, so if you are trying to access the same SQLite database in parallel that would probably be unsafe and cause errors?

nextgenusfs avatar May 19 '23 19:05 nextgenusfs

It appears I had a memory problem as the jobs have been running ok since the memory was increased.

DiegoSafian avatar May 22 '23 09:05 DiegoSafian