Funannotate compare

[danielabermeo8]

Hi, I am new using funannotate and I was trying to compare two annotated genomes with funannotate compare but I got this error. [May 14 06:28 PM]: OS: Ubuntu 20.04, 256 cores, ~ 1057 GB RAM. Python: 3.8.15 [May 14 06:28 PM]: Running 1.8.15 [May 14 06:28 PM]: Now parsing 1 genomes Traceback (most recent call last): File "/root/miniconda3/envs/funannotate/bin/funannotate", line 10, in sys.exit(main()) File "/root/miniconda3/envs/funannotate/lib/python3.8/site-packages/funannotate/funannotate.py", line 716, in main mod.main(arguments) File "/root/miniconda3/envs/funannotate/lib/python3.8/site-packages/funannotate/compare.py", line 265, in main genomeStats = lib.genomeStats(GBK) File "/root/miniconda3/envs/funannotate/lib/python3.8/site-packages/funannotate/library.py", line 9174, in genomeStats from Bio.SeqUtils import GC ImportError: cannot import name 'GC' from 'Bio.SeqUtils' (/root/miniconda3/envs/funannotate/lib/python3.8/site-packages/Bio/SeqUtils/init.py)

So I run funannotate check --show-versions to check what was happening and I got this. Also I enter de PATH for genemark but in this part shows that gmes_petap.pl not installed. I tried everything to download de ete3 package and genemark.

Checking dependencies for 1.8.15

You are running Python v 3.8.15. Now checking python packages... biopython: 1.83 goatools: 1.3.11 matplotlib: 3.4.3 natsort: 8.4.0 numpy: 1.24.4 pandas: 1.5.3 psutil: 5.9.8 requests: 2.31.0 scikit-learn: 1.3.2 scipy: 1.10.1 seaborn: 0.13.2 All 11 python packages installed

You are running Perl v b'5.032001'. Now checking perl modules... Carp: 1.50 Clone: 0.46 DBD::SQLite: 1.72 DBD::mysql: 4.046 DBI: 1.643 DB_File: 1.858 Data::Dumper: 2.183 File::Basename: 2.85 File::Which: 1.24 Getopt::Long: 2.54 Hash::Merge: 0.302 JSON: 4.10 LWP::UserAgent: 6.67 Logger::Simple: 2.0 POSIX: 1.94 Parallel::ForkManager: 2.02 Pod::Usage: 1.69 Scalar::Util::Numeric: 0.40 Storable: 3.15 Text::Soundex: 3.05 Thread::Queue: 3.14 Tie::File: 1.06 URI::Escape: 5.17 YAML: 1.30 local::lib: 2.000029 threads: 2.25 threads::shared: 1.61 All 27 Perl modules installed

Checking Environmental Variables... $FUNANNOTATE_DB=/home/juan.baez/DBermeo/Fdatabase $PASAHOME=/root/miniconda3/envs/funannotate/opt/pasa-2.5.3 $TRINITY_HOME=/root/miniconda3/envs/funannotate/opt/trinity-2.8.5 $EVM_HOME=/root/miniconda3/envs/funannotate/opt/evidencemodeler-1.1.1 $AUGUSTUS_CONFIG_PATH=/root/miniconda3/envs/funannotate/config/ $GENEMARK_PATH=/home/juan.baez/DBermeo/gmes_linux_64_4/gmes_petap.pl All 6 environmental variables are set

Checking external dependencies... Traceback (most recent call last): File "/root/miniconda3/envs/funannotate/bin/ete3", line 5, in from ete3.tools.ete import main File "/root/miniconda3/envs/funannotate/lib/python3.8/site-packages/ete3/tools/ete.py", line 55, in from . import (ete_split, ete_expand, ete_annotate, ete_ncbiquery, ete_view, File "/root/miniconda3/envs/funannotate/lib/python3.8/site-packages/ete3/tools/ete_view.py", line 48, in from .. import (Tree, PhyloTree, TextFace, RectFace, faces, TreeStyle, CircleFace, AttrFace, ImportError: cannot import name 'TextFace' from 'ete3' (/root/miniconda3/envs/funannotate/lib/python3.8/site-packages/ete3/init.py) PASA: 2.5.3 CodingQuarry: 2.0 Trinity: 2.8.5 augustus: 3.5.0 bamtools: bamtools 2.5.1 bedtools: bedtools v2.31.1 blat: BLAT v37x1 diamond: 2.1.9 emapper.py: 2.1.12 exonerate: exonerate 2.4.0 fasta: 36.3.8g glimmerhmm: 3.0.4 gmap: 2024-03-15 hisat2: 2.2.1 hmmscan: HMMER 3.4 (Aug 2023) hmmsearch: HMMER 3.4 (Aug 2023) java: 17.0.3-internal kallisto: 0.46.1 mafft: v7.525 (2024/Mar/13) makeblastdb: makeblastdb 2.15.0+ minimap2: 2.28-r1209 pigz: 2.8 proteinortho: 6.3.1 pslCDnaFilter: no way to determine salmon: salmon 0.14.1 samtools: samtools 1.18 snap: 2006-07-28 stringtie: 2.2.1 tRNAscan-SE: 2.0.12 (Nov 2022) tantan: tantan 49 tbl2asn: 25.8 tblastn: tblastn 2.15.0+ trimal: trimAl v1.4.rev15 build[2013-12-17] trimmomatic: [0.001s][warning][os,container] Duplicate cpuset controllers detected. Picking /sys/fs/cgroup/cpuset, skipping /sys/fs/cgroup/cpuset. ERROR: ete3 not installed ERROR: gmes_petap.pl not installed ERROR: signalp not installed

[ruthpg]

Hi! I just ran into the same issue myself, I kept getting this error with 'GC' from 'Bio.SeqUtils'. Apparently this issue was already known and was answered here --> https://github.com/nextgenusfs/funannotate/issues/1000 and it has to do with the version of biopython you are using and you likely need to change it to a previous version

For me the issue was solved by running the following command: pip install biopython==1.78