GeneLab_Data_Processing
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[Microarray] Unintentional renaming of columns causes issues later in selection of columns
Description
The following error occurred when rendering Affymetrix.qmd for one dataset:
Error in (function (cond) :
error in evaluating the argument 'x' in selecting a method for function 'as.data.frame': Problem while computing `Group.Mean_(1G) = rowMeans(dplyr::select(.,
all_of(current_samples)))`.
Caused by error:
! error in evaluating the argument 'x' in selecting a method for function 'rowMeans': Problem while evaluating `all_of(current_samples)`.
In this particular dataset, some columns were unintentionally renamed because they happen to contain the substring that's being replaced (for other columns), causing this error when trying to select them later on.
Solution
Be more explicit about which columns we want to rename using rename_with() here in Affymetrix.qmd:
df_interim <- df_interim %>% dplyr::rename_with(reformat_names, .cols = matches('\\.condition'), group_name_mapping = design_data$mapping)
The same can be done here for Agile1CMP.qmd to prevent something similar from happening in the future:
df_interim <- df_interim %>% dplyr::rename_with(reformat_names, .cols = matches('\\.condition|^Genes\\.'), group_name_mapping = design_data$mapping)