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Assembly Based ReAligner

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Exception in thread "main" java.lang.IllegalStateException: Unable to delete: abra_temp_dir at abra.ReAligner.init(ReAligner.java:1102) at abra.ReAligner.reAlign(ReAligner.java:121) at abra.ReAligner.run(ReAligner.java:1240) at abra.Abra.main(Abra.java:12) please help me!!!

Hi, I tested building on following 2 systems and get same resolve errors at bottom for build. 1. Centos 7 Apache Maven 3.6.3 (cecedd343002696d0abb50b32b541b8a6ba2883f) Maven home: /export/home/bwiley4/tools/apache-maven-3.6.3 Java version: 11.0.10,...

Per email discussion, this is a Dockerfile that would build whatever the latest ABRA checked out of github.

Is it possible to get as output the assembled contig against which the realignment is done for each targeted region?

Dear all, I have problems with header after realignment of abra tool. Do you have same problem? FixMateInformation doesn't work. Thank you Filip

Hi, I am trying this tool to do realignment in a few regions, but got following error: java.lang.NegativeArraySizeException at java.lang.AbstractStringBuilder.(AbstractStringBuilder.java:64) at java.lang.StringBuffer.(StringBuffer.java:108) at abra.CompareToReference2.getSequence(CompareToReference2.java:400) at abra.KmerSizeEvaluator.getBases(KmerSizeEvaluator.java:44) at abra.KmerSizeEvaluator.identifyMinKmer(KmerSizeEvaluator.java:98) at abra.NativeAssembler.assembleContigs(NativeAssembler.java:273)...

Hi Looks like a great tool I just wondered it will work with amplicon data. I seem to recall haplotype caller struggling with the lack of sequence diversity. Thanks Matt

The output of ABRA is, unfortunately, not necessarily coordinate-sorted, such that I would prefer to replace any usage with one that piped to samtools #sort. In addition, it would be...

Hi I realigned my reads using abra and wants to do somatic calling using Cadabra but keep having a problem and I do not understand why. Here is my command:...

ABRA version - 0.96 Platform - CentOS command used to run ABRA `java -Xmx4G -jar abra-0.96-SNAPSHOT-jar-with-dependencies.jar --in C26.bam --out C26.ABRA.bam --ref hs37d5.fa --targets Agilent_Nimblegen.bed --threads 4 --working temp_dir >abra.log 2>...