RagTag
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Troubling in patching?
Hi,
First thanks for this great tool.
I had get a scaffold sequence that some of its contain N bases, called A.scaffold.
and I used contigs from nanopore using 'Necat' tools, called B.contig.
and ran ragtag.py patch --fill-only --aligner minimap2 --mm2-params '-x asm20' A.scaffold B.contig.
The command line would go through without error, and I got output C.patch.fasta
I found that the size of C.patch.fasta always less than A.scaffold?
Is there something important I got missing?
I mean that if gaps in A.scaffold had be filling, the sequences size of A.scaffold should be less than C.patch.fasta.
Thanks