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Blasr failed

Open arunprasanna83 opened this issue 7 years ago • 1 comments

HALC fails at BLASR step. The installed BLASR version is 3.5.2. Here is the output log:

WARNING: ./temp/step2 was found. Automatically started from step2 2018-09-28 17:14:37.901244

/////STEP 2 STARTED////////////////////////////////////////////////////////////////////////////////////////////////// Running command: blasr ./temp/step1/pb-001.fa /scratch/dragon/amd/nagaraap/ERROR_CORRECT/barcoded_10XSR.fasta -m 5 --out ./temp/step2/blasrresult-001.m5 --maxScore 2000 --minMatch 8 --minAlnLength 300 --nCandidates 30 --bestn 20 --nproc 16 1>./temp/step2/blasr_1.out 2>./temp/step2/blasr_1.err ERROR: Failed to run BLASR:Unknown error 256

Please let me know the issue here.

arunprasanna83 avatar Sep 28 '18 15:09 arunprasanna83

Same error`s at Step 2. BLASR version 5.3.2.

[vk@GenomeBiology work]$ python ~/distr/halc/runHALC.py d_PB.fa short_reads_A.fasta -t 22 -r
2018-11-02 16:26:58.353467

/////STEP 1 STARTED//////////////////////////////////////////////////////////////////////////////////////////////////
Running command: Chunker -s 200M -o ./temp/step1/pb-%03d.fa d_PB.fa 1>./temp/step1/SeqChunker.out 2>./temp/step1/SeqChunker.err
25 files created

/////STEP 1 DONE/////////////////////////////////////////////////////////////////////////////////////////////////////

/////STEP 2 STARTED//////////////////////////////////////////////////////////////////////////////////////////////////
Running command: blasr ./temp/step1/pb-001.fa short_reads_A.fasta -m 5 --out ./temp/step2/blasrresult-001.m5 --maxScore 2000 --minMatch 8 --minAlnLength 300 --nCandidates 30 --bestn 20 --nproc 22 1>./temp/step2/blasr_1.out 2>./temp/step2/blasr_1.err
ERROR: Failed to run BLASR: Unknown error 256

[vk@GenomeBiology work]$ more temp/step2/blasr_1.out 
ERROR! Reading fasta files greater than 4Gbytes is not supported.

-rw-rw-r--. 1 vk vk 203G ноя 2 15:48 short_reads_A.fasta -rw-rw-r--. 1 vk vk 4,9G окт 26 12:53 d_uni_PB.fa

Is there any solution

VoronDM avatar Nov 02 '18 15:11 VoronDM