ComplexHeatmap
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Unable to use_raster function while plotting heatmap for CopyKit
Hi,
I am using CopyKit to analyze my single cell DNA data. I have merged 7 objects containing about 25k cells. I want to plot a combined heatmap for the same but am unable to do so using the following function:
pdf(glue("{outputdir}/allaneuploid_ht.pdf", height = 10, width = 8))
print(plotHeatmap(merged_ckobj_cbs[,colData(merged_ckobj_cbs)$is_aneuploid==T], col = col_fun, order_cells = "hclust", n_threads = 100))
dev.off()
I get the following error:
Error in Cairo(width, height, type = "png", file = filename, pointsize = pointsize, :
Graphics API version mismatch
Error: The size of the temporary image for rasterization is too huge (12167 x 876 px) that it is cannot be handled by the device function Cairo:CairoPNG(). Please reduce the maximal size of temporary image by
setting proper values for ht_opt$raster_temp_image_max_width and ht_opt$raster_temp_image_max_height.
> sessionInfo()
R version 4.4.0 (2024-04-24)
Platform: x86_64-pc-linux-gnu
Running under: CentOS Linux 7 (Core)
Matrix products: default
BLAS/LAPACK: /omics/odcf/analysis/OE0585_projects/chromothripsis/urja_python/conda_environments/my_r_env/lib/libopenblasp-r0.3.21.so; LAPACK version 3.9.0
locale:
[1] LC_CTYPE=en_US.UTF-8 LC_NUMERIC=C LC_TIME=en_US.UTF-8 LC_COLLATE=en_US.UTF-8 LC_MONETARY=en_US.UTF-8 LC_MESSAGES=en_US.UTF-8 LC_PAPER=en_US.UTF-8 LC_NAME=C
[9] LC_ADDRESS=C LC_TELEPHONE=C LC_MEASUREMENT=en_US.UTF-8 LC_IDENTIFICATION=C
time zone: Europe/Berlin
tzcode source: system (glibc)
attached base packages:
[1] stats4 stats graphics grDevices utils datasets methods base
other attached packages:
[1] magick_2.8.3 glue_1.8.0 lubridate_1.9.3 forcats_1.0.0 stringr_1.5.1 dplyr_1.1.4 purrr_1.0.2 readr_2.1.5
[9] tidyr_1.3.1 tibble_3.2.1 ggplot2_3.5.1 tidyverse_2.0.0 BiocParallel_1.38.0 magrittr_2.0.3 copykit_0.1.3 DNAcopy_1.78.0
[17] Rsubread_2.16.1 SingleCellExperiment_1.26.0 SummarizedExperiment_1.34.0 Biobase_2.64.0 GenomicRanges_1.56.0 GenomeInfoDb_1.40.1 IRanges_2.38.0 S4Vectors_0.42.0
[25] BiocGenerics_0.50.0 MatrixGenerics_1.16.0 matrixStats_1.4.1
loaded via a namespace (and not attached):
[1] RColorBrewer_1.1-3 rstudioapi_0.16.0 jsonlite_1.8.9 shape_1.4.6.1 modeltools_0.2-23 farver_2.1.2 GlobalOptions_0.1.2 fs_1.6.5 zlibbioc_1.50.0
[10] vctrs_0.6.5 ggtree_3.12.0 htmltools_0.5.8.1 S4Arrays_1.4.1 BiocNeighbors_1.22.0 SparseArray_1.4.8 gridGraphics_0.5-1 htmlwidgets_1.6.4 plotly_4.10.4
[19] igraph_2.0.3 mime_0.12 lifecycle_1.0.4 iterators_1.0.14 pkgconfig_2.0.3 Matrix_1.7-0 R6_2.5.1 fastmap_1.2.0 GenomeInfoDbData_1.2.12
[28] shiny_1.8.1.1 clue_0.3-65 digest_0.6.36 aplot_0.2.2 colorspace_2.1-1 ggnewscale_0.5.0 patchwork_1.3.0 timechange_0.3.0 fansi_1.0.6
[37] httr_1.4.7 abind_1.4-8 compiler_4.4.0 withr_3.0.2 doParallel_1.0.17 viridis_0.6.5 MASS_7.3-60.2 DelayedArray_0.30.1 rjson_0.2.23
[46] bluster_1.14.0 gtools_3.9.5 tools_4.4.0 ape_5.8 prabclus_2.3-4 httpuv_1.6.15 nnet_7.3-19 dbscan_1.1-12 nlme_3.1-165
[55] promises_1.3.0 grid_4.4.0 cluster_2.1.6 generics_0.1.3 gtable_0.3.6 tzdb_0.4.0 class_7.3-22 hms_1.1.3 data.table_1.16.2
[64] utf8_1.2.4 XVector_0.44.0 flexmix_2.3-19 foreach_1.5.2 pillar_1.9.0 yulab.utils_0.1.7 later_1.3.2 robustbase_0.99-4-1 circlize_0.4.16
[73] splines_4.4.0 treeio_1.28.0 lattice_0.22-6 survival_3.7-0 tidyselect_1.2.1 ComplexHeatmap_2.20.0 miniUI_0.1.1.1 gridExtra_2.3 mixtools_2.0.0
[82] diptest_0.77-1 scquantum_1.0.0 DEoptimR_1.1-3-1 stringi_1.8.4 UCSC.utils_1.0.0 lazyeval_0.2.2 ggfun_0.1.5 codetools_0.2-20 kernlab_0.9-33
[91] ggplotify_0.1.2 cli_3.6.3 uwot_0.2.2 xtable_1.8-4 segmented_2.1-1 munsell_0.5.1 Rcpp_1.0.13 png_0.1-8 parallel_4.4.0
[100] mclust_6.1.1 ggalluvial_0.12.5 viridisLite_0.4.2 tidytree_0.4.6 scales_1.3.0 crayon_1.5.3 fpc_2.2-13 GetoptLong_1.0.5 rlang_1.1.4
I got a similar error on Ubuntu 18, but I successfully plotted this heatmap using Windows. It might be related to the API of some functions on Linux.