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Something to share regarding multiple samples with CellChat
Based on my experience, I suggest running CellChat on separate samples when having multiple biological replicates. I found that even the most frequent interactions observed across samples in a group often appear only in specific samples. Running CellChat on a merged dataset may overestimate the importance of these interactions.
@NPTL1201 You can set the parameter 'min.samples' when running filterCommunication, leading to robust identification across multiple replicates.